The Experimental Design Assistant (EDA) is a free online software that helps researchers design robust in vivo experiments that are more likely to give reproducible results. This interactive virtual workshop on using the EDA will be helpful for anyone involved in planning and designing experiments or teaching experimental design. During the session Dr Esther Pearl (NC3Rs Programme Manager for Experimental Design) will give a live demonstration of the EDA before answering your questions and providing personalised feedback as you design your own experiment.